Located in Santa Cruz Island, the second largest in the Galapagos, Puerto Ayora is located in the southeast of this large, round volcanic island is the economic center of the Islands. It has the largest population among the four inhabited islands (approx. 18,000), which economically relies mostly on tourism—including refurbishing and resupplying Galapagos cruise yachts—along with fishing and boat-building.

Puerto Ayora is home to both the Galapagos National Park Service Headquarters and Charles Darwin Research Station, the center of the great restorative efforts taking place in the park, a UNESCO World Heritage site. Here we go ashore to visit the Giant Tortoise Breeding and Rearing Program run by the research station, which began by rescuing the remaining 14 tortoises on the island of Española in 1970. This program has restored the population of animals there to over 1,000 today. You will see many of these animals, with their sweet ET necks and faces; from hatchlings to juveniles to large, distinguished individuals like Lonesome George, the last of his particular race of tortoises. The local color of this port makes for an attractive stop-off, with restaurants, souvenir shops, and internet cafés