Jukani Wildlife Sanctuary is home to big cats such as lions (white and tawny), cheetahs, tigers (Siberian, Bengal and white), leopard (spotted and black), jaguar, pumas, caracal, serval cats and other wildlife species such as zebra, wild dog, springbuck, zorilla, honey badger, raccoons and various snake species (which are housed separately).

At Jukani the focus is on conservation education and specifically the plight of large predators in captivity, in South Africa and all over the world. “Big cats” are extraordinary animals with a dangerous beauty that is both awe inspiring but also fearsome because of their power and ability to kill. However at most wildlife facilities various wildlife species and especially large predatory species are kept as a way of attracting visitors without regard for the animals' physical and emotional needs, they are just commodities to attract visitors. The Jukani approach is one of respect for what each animal represents. All the female predator cats are on birth control, and the sanctuary is a no-touch facility.